Research Article
Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation
Table 3
The levels of IL-2, IL-10, and IFN-γ in plasma of BN recipients rats 7 days after liver transplantation.
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The recombination vector of lentivirus contains shRNA targeting the 4-1BB gene was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The blood samples were gathered 7 days after liver transplantation. The concentrations of interleukin-2 (IL-2), IL-10, and interferon- (IFN-) in plasma were tested using enzyme-linked immunosorbent assay (ELISA). Model: liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB-Lentivector1 group. Data were expressed as means ± SD, . #5 compared with the sham (control) group; * compared with the LV-NC group. |