BioMed Research International / 2013 / Article / Tab 3

Research Article

Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

Table 3

The levels of IL-2, IL-10, and IFN-γ in plasma of BN recipients rats 7 days after liver transplantation.

GroupsIL-2 (pg/mL)IL-10 (pg/mL)IFN- (pg/mL)

Sham (Control)71.32 ± 8.6716.85 ± 2.0352.49 ± 6.75
Model139.15 ± 13.73#15.47 ± 1.27 86.2 ± 8.27#
LV-NC142.86 ± 16.5818.35 ± 2.0680.3 ± 11.64
LV-sh4-1BB178.05 ± 8.64*16.59 ± 1.8557.86 ± 8.33*

The recombination vector of lentivirus contains shRNA targeting the 4-1BB gene was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The blood samples were gathered 7 days after liver transplantation. The concentrations of interleukin-2 (IL-2), IL-10, and interferon- (IFN- ) in plasma were tested using enzyme-linked immunosorbent assay (ELISA). Model: liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB-Lentivector1 group. Data were expressed as means ± SD, . # 5 compared with the sham (control) group; * compared with the LV-NC group.