Demethylation of Cancer/Testis Antigens and CpG ODN Stimulation Enhance Dendritic Cell and Cytotoxic T Lymphocyte Function in a Mouse Mammary Model
In vitro cytotoxic activity of P1A-specific CTLs on H-2Ld-restricted 4T1 cells. 4T1 tumor cells were treated with 5-aza for 24 h and then cultured with P1A-specific CTLs at different effector/target ratios for 16 h. The number of apoptotic 4T1 cells (Annexin V positive/7-AAD (early apoptosis) cells) increased in a dose-dependent manner after incubation with P1A-specific peptide + CpG ODN-treated P1A-specific CTLs. The differences were significant () compared to the P1A-specific peptide or CpG ODN alone. CTLs did not cause similar apoptosis in control group, even if at a higher effect/target ratio of 25 : 1.
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