Induction of Mast-Cell Accumulation by Promutoxin, an Arg-49 Phospholipase A₂

Table 2

The influence of blocking antibodies (Ab) against cell-adhesion molecules on promutoxin- (5.0 μg) induced mast-cell accumulation in mouse peritoneum.

Compound injected	Number of mast cells; (×10^3)
Saline	14.6 (6.1–23)
Promutoxin	44.6 (21.4–60.8)
L-selectin Ab + promutoxin	38.0 (18.6–69.0)
LFA-1 Ab + promutoxin	20.6 (9.1–40)*
CD18 Ab + promutoxin	5.7 (3.1–11.7)*
ICAM-1 Ab + promutoxin	10.4 (2.9–14.5)*
Hamster IgG1 + promutoxin	39.8 (17.0–63.1)
Rat IgG2a + promutoxin	43.3 (19.3–58.0)

The values shown are medians (range) for six separate experiments. Monoclonal antibodies (all at a dose of 1 mg·kg⁻¹) against the adhesion molecules L-selectin, LFA-1, CD18 and ICAM-1 were intravenously injected, respectively, for 30 min before intraperitoneal injection of 5 μg promutoxin for 6 h. ^*𝑃<0.05 compared with the response to promutoxin alone.