Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit

Table 1

Table summarizing results from the analyses of conduits at different distances from the bladder junction. Animals numbered 1–4 were transplanted with minced urothelial particles, and animals 1 and 4 underwent radiological assessment before termination. At the junction, (0 cm) part of the native bladder was included in the biopsy. Biopsies from conduit running through the abdominal muscle (3 cm) reveal a median urothelial lining of 6 mm, SD 2.5 mm (33 ± 13%). Biopsy at the boarder between abdominal muscle and subcutaneous fat (5 cm) demonstrates a median urothelial lining of 6 mm, SD 6 mm (33 ± 33%).

Pig		Weeks after transplantation	0 cm; (mm)	3 cm; (mm)	3 cm; (%)	5 cm; (mm)	5 cm; (%)	8 cm; (mm)	Inner lumen (mm)
1	X-ray	4	18	11	61	+	+	+	18
2		4	18	4	22	+	+	+	18
3		4	18	6	33	17	89	+	18
4	X-ray	5	18	3	17	8	44	+	18
5	Control	4	16	0	0	0	0	0	18
6	Control	5	16	0	0	0	0	0	18

All transplanted conduits demonstrated some urothelial cells at the most distal part of the conduit but none in controls. Marking with (+) indicates the presence of urothelial cells but not as confluent epithelium at the inner luminal surface.