Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Figure 2

PCR products on 1,5% agarose gel. M: molecular weight marker 100 bp to 10000 bp (Jena Bioscience, Germany). Lane 1: negative control, lane 2: 5 μL of viral RNA (from 3000 ng of purified TrV), lane 3: 5 μL of viral RNA (from 300 ng of purified TrV), lane 4: 5 µL of viral RNA (from 30 ng of purified TrV), lane 5: 5 μL of viral RNA (from 0,03 ng of purified TrV), lane 6: 5 μL of viral RNA (from 1 mg of feces), lane 7: 5 μL of viral RNA (from 0.5 mg of feces), lane 8: 5 μL of viral RNA (from 0,25 mg of feces), lane 9: 5 μL of feces samples (without RNA extraction), lane 10: 3 μL of feces samples (without RNA extraction), and lane 11: 1 μL of feces samples (without RNA extraction).