Figure 2: Spheroid formation of HTR8/SVneo and JEG3 cells via hanging drops. Forty hanging drops per cell line were produced with 20 000 cells per 30 μL drop. The environment of the hanging drop delivers the prerequisite for spheroid formation. After an incubation period of 48 h, 100% of HTR8/SVneo-containing drops formed visible spheroids (a, b), while only 50% of JEG3-containing drops formed spheroids (c). JEG3 spheroids appeared smaller in circumference, less globular (oblong shapes), and rather on the verge of disaggregation (d).