Viability Reduction and Rac1 Gene Downregulation of Heterogeneous Ex-Vivo Glioma Acute Slice Infected by the Oncolytic Newcastle Disease Virus Strain V4UPM
Figure 1
The figure summarises the protocol for preparation of EGAS cores. Surgical specimens obtained from patient (a) were immediately transferred to a petri dish containing ice-cold aCSF bubbling with filtered carbogen gas. (b) The specimen was prepared by punch biopsy and sliced with a sterile blade to form rounded glioma cores. (c) The cores were later placed into a 6-well plate for acute infection with NDV. 2 cores per group were collected from each patient ().
