Interleukin-33 Drives Activation of Alveolar Macrophages and Airway Inflammation in a Mouse Model of Acute Exacerbation of Chronic Asthma

Table 2

Expression of proinflammatory cytokine mRNA and protein by IL-33-stimulated MH-S cells.

Cytokine	Expression of mRNA (relative to HPRT × 10^3)		Concentration of protein in culture supernatant
	Unstimulated	IL-33 stimulated	Unstimulated	IL-33 stimulated
TNF-𝛼	3.43 ± 0.33	6.72 ± 0.86**	197.3 ± 38.2	398.3 ± 23.8**
IL-1𝛽	0.66 ± 0.04	3.88 ± 0.48***	51.6 ± 28.4	93.1 ± 9.7
IL-6	9.9 ± 6.2	85.5 ± 9.5***	124.0 ± 8.5	709.7 ± 38.8***
CXCL-1	0.001 ± 0.000	0.002 ± 0.000**	10.6 ± 0.4	19.2 ± 0.6***
IL-12p40	0.003 ± 0.000	0.228 ± 0.004***	11.2 ± 1.4	49.2 ± 0.6***
G-CSF	0.001 ± 0.000	0.004 ± 0.001	87.9 ± 8.5	538.5 ± 33.2***

Data are mean ± SEM (𝑛= 4–6 replicates per group). Significant differences relative to unstimulated control are shown as **(𝑃<0.01) and ***(𝑃<0.001).