Degradation of Diuron by Phanerochaete chrysosporium: Role of Ligninolytic Enzymes and Cytochrome P450

Table 3

Degradation of diuron by enzymatic crude filtrate from P. chrysosporium cultures after 24 h.

Treatment	Veratryl alcohol; (0.5 mM)	H2O2; (0.1 mM)	MnSO4; (0.5 mM)	Crude filtrate*	Recupered diuron (μg/mL)
Control	−	−	−	−	6.47±0.43
1	+	+	+	+	7.40±0.46
2	−	+	−	+	6.34±0.72
3	+	+	−	+	5.98±0.35
4	−	+	+	+	6.27±0.31
5	+	−	−	+	5.66±0.48
6^#	+	+	+	+	7.39±0.39

 *40.0 U/L of lignin peroxidase activity and 50 U/L of manganese peroxidase activity. All the treatment contained diuron (7 μg/mL) and sodium malonate buffer 50 mM, pH 4.5. Control was run only with diuron and buffer. ^#Treatment 6 was performed using boiled crude enzyme. Degradation values are means ± SD (𝑛=3).