Constitutive Optimized Production of Streptokinase in Saccharomyces cerevisiae Utilizing Glyceraldehyde 3-Phosphate Dehydrogenase Promoter of Pichia pastoris
Figure 2
Screening of S. cerevisiae transformants. (a) S. cerevisiae transformed with pB2ZB2-SK expression vector were selected on YPD-Zeocin (200 g/mL) medium. Transformants with pB2ZB2-SK vector (Lane 1), untransformed S. cerevisiae (Lane 2). (b) Colony PCR (P3 and P4 primers) of Saccharomyces transformants selected on YPD Zeocin medium. Untransformed S. cerevisiae (Lane 1), transformants with 1.5 Kbp skc expression cassette fragments (Lanes 2, 3, 4, and 5) and 0.25–12.0 Kbp DNA ladder (Lane 6).
