Constitutive Optimized Production of Streptokinase in Saccharomyces cerevisiae Utilizing Glyceraldehyde 3-Phosphate Dehydrogenase Promoter of Pichia pastoris
Figure 3
(a) Plasminogen activation assay of rSK. Growth profile and outline of SK expression of S. cerevisiae clone. Cells were grown in YPD medium for 5 days. Aliquots of cells were examined at different time intervals for growth and SK activity in the cell lysis supernatant by chromogenic assay using S-2251 substrate. (b) Expression of rSK has no toxic effect on the growth rate of the S. cerevisiae host. S. cerevisiae INVSc1 cells were transformed with parent plasmids and recombinant expression vector pB2ZB2-SK. Cells were cultured in YPD broth and aliquots were withdrawn at indicated time points up to 120 hrs, OD600 recorded and plotted. The above data is a mean of three to six independent experiments.
