Constitutive Optimized Production of Streptokinase in Saccharomyces cerevisiae Utilizing Glyceraldehyde 3-Phosphate Dehydrogenase Promoter of Pichia pastoris
Figure 4
Southern blot analyses of S. cerevisiae-SK clone. Genomic 635 DNA of the clone was restricted with SapI/AgeI enzymes, blotted, and hybridized with α32P-labelled 1.63 Kbp PGAP-skc-AOXTT cassette fragment. S. cerevisiae clone genomic DNA extracted at 24 (Lane 2), 72 (Lane 3), 96 (Lane 4), and 120 hrs (Lane 5) and untransformed S. cerevisiae genomic DNA (Lane 1). 1.63 Kbp PGAP-skc-AOXTT cassette fragment is specified.
