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BioMed Research International
Volume 2013, Article ID 270805, 4 pages
http://dx.doi.org/10.1155/2013/270805
Research Article

A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes

1Biomedical Engineering Department, College of Engineering, Peking University, Beijing 100871, China
2Center for Bioinformatics, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
3National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China
4Institute of Computer Science and Technology, Peking University, Beijing 100871, China

Received 4 July 2013; Accepted 13 September 2013

Academic Editor: Yi Zhao

Copyright © 2013 Ming Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database.