Secretion of Biologically Active Heterologous Oxalate Decarboxylase (OxdC) in Lactobacillus plantarum WCFS1 Using Homologous Signal Peptides

Table 1

Bacterial strains and plasmids used in this study.

Strains and plasmids	Characteristics^a	Source/references
Strains
E. coli DH10B	Host strain for cloning commercial	Invitrogen
L. plantarum WCFS1*	Host strain, plasmid-free, silage isolate	Kleerebezem et al. (2003) [25]
Plasmid
pLp_0373sAmyA	p256/pUC(pGEM)ori; PsppA; Lp_0373; amyA; Em^r	Mathiesen et al. (2009) [24]
pLp_3050sAmyA	p256/pUC(pGEM)ori; PsppA; Lp_3050; amyA; Em^r	Mathiesen et al. (2009) [24]
pLB36	pET 32a oxdC; Am^r	Just et al. (2004) [26]
pSip-OxdC	p256/pUC(pGEM)ori; PsppA; oxdC; Em^r	Kolandaswamy et al. (2009) [23]
pLp_0373sOxdC	p256/pUC(pGEM)ori; PsppA; spLp_0373 fused to the oxdC; Em^r	This work
pLp_3050sOxdC	p256/pUC(pGEM)ori; PsppA; spLp_3050fused to the oxdC; Em^r	This work

aFor strains, genotypic and phenotypic characteristics are given; for plasmid, plasmid and cloned-cassette characteristics are given; Em^r, Am^r: resistance to erythromycin and ampicillin, respectively.
∗L. plantarum WCFS1 is a single colony isolate of strain NCIM8826 (Kleerebezem et al., 2003 [25]).