Secretion of Biologically Active Heterologous Oxalate Decarboxylase (OxdC) in Lactobacillus plantarum WCFS1 Using Homologous Signal Peptides

Table 3

Oxalate decarboxylase (OxdC) activity in concentrated culture supernatant and cell lysate of various recombinant L. plantarum WCFS1 strains^a.

Name of strain (plasmid)	Protein localization	OxdC (U/mg protein) activity in culture supernatant at OD600 = 1.7	OxdC (U/mg protein) activity in cell fraction at OD600 = 1.7	Secretion efficiency* (%)
WCFS1 (Control)	NP	NA	NA	ND
WCFS1 (pSIP-OxdC)	Intracellular	NA	2.8±0.2	ND
WCFS1 (pLp_0373sOxdC)	Extracellular	0.05±0.01	0.18±0.07	22
WCFS1 (pLp_3050sOxdC)	Extracellular	0.02±0.01	0.21±0.07	9

aData are mean of three independent experiments, NP: no production, NA: no activity, and ND: not determined, and ∗ secretion efficiency were determined at OD₆₀₀ = 1.7 between the culture supernatant and cell fraction of respective strains.