Research Article

Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure

Figure 4

Representative gelatin zymograms of porcine homogenates from LV myocardium with proteolytically active bands of 68, 72, and 115 kDa at baseline (line 1), after the depletion of active gelatinolytic forms using gelatin-Sepharose 4B affinity chromatography (line 2), and subsequently after the 24-hour incubation at 4°C (line 3). Line 4—positive control, the culture media from canine macrophage-like DH82 cell line.
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