Analysis of Activated Platelet-Derived Growth Factor β Receptor and Ras-MAP Kinase Pathway in Equine Sarcoid Fibroblasts

Table 1

Primers sets used to amplify cDNA from exons 11–20 of PDGFβR in EqS cells.

Primers sets	Annealing position	Annealing 𝑇^∘	Fragments size
F1: ATCCTCATCATGCTCTGGCAG	F1: 2119	52°C	628 bp
R1: TCTCCTTTCATGTCCAGCATG	R1: 2747
F1: ATCCTCATCATGCTCTGGCAG	F1: 2119	52°C	1226 bp
R3: TCGAGAAGCAGCACCAGCTG	R3: 3344
F3: TGGCTACATGGACATGAGCAAG	F3: 2682	52°C	663 bp
R3: TCGAGAAGCAGCACCAGCTG	R3: 3344
F3: TGGCTACATGGACATGAGCAAG	F3: 2682	52°C	593 bp
R2: ATCTCGTAGATCTCGTCGGAG	R2: 3275

𝑇^∘: temperature; F: forward; R: reverse; bp: base pairs.