Digital Microscopy Assessment of Angiogenesis in Different Breast Cancer Compartments

Table 4

Steps in evolution of vasculature assessment.

Study	Region measured	Parameter recommended	Measured by
Weidner et al. 1991 [7]	Hot spot at tumor border	MVD	human expert
Barbareschi et al. 1995 [25], Simpson et al. 1996 [43], Schoell et al. 1997 [44], Fridman et al. 2000 [45]	Hot spots identified on low magnification	EA, MVD	CIAS
Belien et al. 1999 [29]	Whole slide + hotspot	MVD	CIAS
Oh et al. 2001 [42]	Random spots	MVD	CIAS
Kim et al. 2003 [31]	Whole slide + hotspot	MVD + EA	CIAS
Chantrain et al. 2003 [32]	Entire sample	EA	CIAS
Mikalsen et al. 2011 [33]	Hot spots identified on low magnification	MVD	CIAS
Our method	Whole slide + domain specific large hot spots	EA	CIAS + human expert