Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses

Table 2

Detection of HeV in experimentally infected horses.

Day	Microsphere array assay
	Henipa N	Henipa P	qPCR (^a)	LAMP (^a)
	(MFI)	(MFI)	(Ct)	(U/Pos)
Horse 1
0	148	213	U	U
1	185	256	U	U
2	20 858	23 745	37.5	U
3	22 088	24 593	34.7	Pos
4	22 828	24 049	35.9	Pos
5	23 734	24 985	29.5	Pos
6	23 856	25 197	32.8	Pos
Horse 2
0	219	104	U	U
1	197	296	U	Pos
2	22 304	23 547	36.3	Pos
3	22 983	23 625	32.4	Pos
4	11 638	8 142	38.9	Pos
5	23 063	24 068	34.3	Pos
6	22 914	23 333	31.1	Pos
7	23 024	24 284	28.1	Pos
8	22 900	24 145	29.2	Pos
9	22 900	24 951	35.2	Pos
Horse 3
0	186	301	U	U
1	221	288	U	U
2	10 734	375	42*	Pos
4	1 271	2 251	41.4*	Pos
5	5 406	7 018	43*	Pos
6	1 804	8 019	U	U
7	16 731	20 575	37.7	Pos
Pos control	23228	23451
NTC	226	298

Comparison of microsphere array assays performed on archival RNA extracted from daily nasal swabs of experimentally infected horses [21].
(^a) Comparison with qPCR and loop-mediated amplification (LAMP) assay results [22] in retrospective analysis. Day indicates sampling day after challenge. MFI: median fluorescence intensity; U: undetected; Pos: positive reaction; *indicates qPCR indeterminate results; NTC: no template control. All positive results are in bold.