Craniosynostosis-Associated Fgfr2C342Y Mutant Bone Marrow Stromal Cells Exhibit Cell Autonomous Abnormalities in Osteoblast Differentiation and Bone Formation
Figure 4
The mutation inhibits mineralized tissue formation in vivo. (a) Radiographic image of representative implants shows increased radiodensity of ossicles formed by wild type (WT), as compared to those formed by Crouzon (Cz) cells. (b) Quantification of radiodense tissue by densitometry confirms that implanted wild type cells formed significantly greater amounts of mineralized tissue compared to mutant cells ( implants per genotype). versus WT. (c) Homogenized implants formed by wild type cells also have significantly greater levels of alkaline phosphatase enzyme expression than homogenized implants formed by Crouzon cells ( implants per genotype). versus WT.
