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BioMed Research International
Volume 2013 (2013), Article ID 294289, 7 pages
http://dx.doi.org/10.1155/2013/294289
Research Article

Antivenom Effects of 1,2,3-Triazoles against Bothrops jararaca and Lachesis muta Snakes

1Programa de Pós-Graduação em Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brazil
2Programa de Pós-Graduação em Biologia das Interações, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brazil
3Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Outeiro de São João Batista, s/n, 3 Andar, Sala 310, 24020-141 Niterói, RJ, Brazil
4Departamento de Química Orgânica, Programa de Pós-Graduação em Química, Universidade Federal Fluminense, Niterói, RJ, Brazil
5Fundação Ezequiel Dias, Centro de Pesquisa e Desenvolvimento, Belo Horizonte, MG, Brazil

Received 24 January 2013; Revised 25 March 2013; Accepted 26 March 2013

Academic Editor: Juergen Buenger

Copyright © 2013 Thaisa F. S. Domingos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Snake venoms are complex mixtures of proteins of both enzymes and nonenzymes, which are responsible for producing several biological effects. Human envenomation by snake bites particularly those of the viperid family induces a complex pathophysiological picture characterized by spectacular changes in hemostasis and frequently hemorrhage is also seen. The present work reports the ability of six of a series of 1,2,3-triazole derivatives to inhibit some pharmacological effects caused by the venoms of Bothrops jararaca and Lachesis muta. In vitro assays showed that these compounds were impaired in a concentration-dependent manner, the fibrinogen or plasma clotting, hemolysis, and proteolysis produced by both venoms. Moreover, these compounds inhibited biological effects in vivo as well. Mice treated with these compounds were fully protected from hemorrhagic lesions caused by such venoms. But, only the B. jararaca edema-inducing activity was neutralized by the triazoles. So the inhibitory effect of triazoles derivatives against some in vitro and in vivo biological assays of snake venoms points to promising aspects that may indicate them as molecular models to improve the production of effective antivenom or to complement antivenom neutralization, especially the local pathological effects, which are partially neutralized by antivenoms.