BioMed Research International / 2013 / Article / Tab 2 / Research Article
A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth Table 2 Cocktail primer list.
Pathogen Targeted gene Primer name Sequence (5′-3′) EHEC stx1 Forward stx1 Biotin-ACAGGATTTGTTAACAGGAC Reverse stx1 Tag1-TCTGTATTTGCCGAAAACGT stx2 Forward stx 2 Biotin-GATACAGAGAGAATTTCGTC Reverse stx 2 Tag1-GCCAGTTATCTGACATTCTG Shigella spp. and EIECipaH Forward ipaHF Biotin-CTCGCAGAGAAACTTCAGCTCT Reverse ipaHR Tag2-TTCTCTTCACGGCTTCTGACCAT Salmonella spp.invA Forward invA Biotin-TGACAGAATCCTCAGTTTTTCA Reverse invA Tag3-AGATAAGACGGCTGGTACTGAT Internal control Forward IPC Biotin-ACTCTTCCTAGCAGGATCCCTCTAAG Reverse IPC Tag4-GCAATTCTAATACGACTCACTATAGG