Research Article

A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth

Table 5

Specificity of CPDC assay.

BacteriaSerotype (Virulence factor) Reacted Dipstick lineCPDC assay (positive/strains)

EHECO26: H- (stx1)Line 14/4
O26: H11(stx1)Line 15/5
O26: H11(stx1/2)Line 13/3
O111: H- (stx1)Line 13/3
O111: H- (stx1/2)Line 12/2
O115: H10(stx1)Line 11/1
O119: H2(stx1)Line 11/1
O121: H19(stx2)Line 14/4
O128: H- (stx1/2)Line 11/1
O157: H7(stx2)Line 110/10
O157: H7(stx1/2)Line 110/10
O157: H-(stx1/2)Line 110/10
EIECO28: H- (IpaH)Line 25/5
O124: H- (IpaH)Line 24/4
O136: H- (IpaH)Line 210/10
O144: H- (IpaH)Line 23/3
O164:H- (IpaH)Line 23/3
Shigella boydii (IpaH)Line 28/8
Shigella dysenteriae (IpaH)Line 216/16
Shigella flexneri (IpaH)Line 213/13
Shigella sonnei (IpaH)Line 27/7
Salmonella enterica
 subsp.enterica serovar Typhimurium (InvA)Line 310/10
 subsp.enterica serovar Typhi (InvA)Line 310/10
 subsp.enterica serovar Enteritidis (InvA)Line 37/7
 subsp.enterica serovar Dublin (InvA)Line 310/10
 subsp.enterica serovar paratyphi A (InvA)Line 33/3
Salmonella bongoriGTC 03793 T(InvA)Line 31/1
Escherichia coli GTC00503 T N*0/1
Escherichia albertiiGTC 16441 T N0/1
Escherichia fergusoniiGTC 01720 T N0/1
Escherichia vulneris GTC 10613 T N0/1
Escherichia hermanniiGTC 10612 T N0/1
Escherichia blattaeGTC 01342 T N0/1
Citrobacter freundiiGTC 14890 T N0/1
Citrobacter diversusGTC 00114 T N0/1
Citrobacter rodentium GTC 14911 T N0/1
Citrobacter youngaeGTC 14914 N0/1
Klebsiella pneumonia GTC 14868 T N0/1
Enterobacter cloacaeGTC 00109 T N0/1
Enterobacter aerogenesGTC 14962 T N0/1
Cronobacter sakazakiiGTC 14952 T N0/1
Serratia marcescensGTC 14672 N0/1
Yersinia enterocoliticaGTC 00127 T N0/1
Vibrio parahaemolyticus GTC02055 N0/1
Pseudomonas aeruginosaGTC 00002 T N0/1
Staphylococcus aureusGTC 00286 T N0/1

N*: no positive line.
Reacted line no. 1 is stx1 and 2 for EHEC, line 2 is ipaH for Shigella spp. and EIEC, and line 3 is for invA for Salmonella.