BioMed Research International / 2013 / Article / Fig 5

Research Article

Defects in Base Excision Repair Sensitize Cells to Manganese in S. cerevisiae

Figure 5

Effect of Mn2+ and Mg2+ on the mutation rate of the reversion of the lys2-10A allele. (a) Mutation rates determination by the yeast mutator assay using a strain carrying the lys2-10A allele was performed in the presence of increasing concentrations of Mn2+. The appearance of Lys+ revertant colonies indicates a mutator phenotype. Rates are calculated as described in Section 2 and standard deviation is included at the top of each bar. (b) Cotreatment with 10 mM Mg2+ protects cells from the toxicity of Mn2+. Survival was determined as described in Section 2. (c) Cotreatment with 10 mM Mg2+ reduces the accumulation of mutations on the lys2-10A allele induced by 1.5 mM Mn2+. Each bar corresponds to the average of three sets of experiments using five independent colonies per set.

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