Defects in Base Excision Repair Sensitize Cells to Manganese in S. cerevisiae

Table 1

Strains used in this study.

Gene	ORF	Function
HSP104	YLL026W	Protein disaggregase
RAD2	YGR258C	Nucleotide excision repair endonuclease
RAD52	YML032C	Homologous recombination
SOD2	YHR008C	Mitochondrial superoxide dismutase
RAD18	YCR066W	Postreplication repair
CTA1	YDR256C	Catalase activity
SOD1	YJR104C	Superoxide dismutase activity
MLH1	YMR167W	Mismatch repair
GSH2	YOL049W	Glutathione synthetase activity
GSH1	YJL101C	Glutamate-cysteine ligase activity
APN1	YKL114C	Base excision repair
UBC13	YDR092W	DNA postreplication repair
RAD27	YKL113C	Base excision repair, DNA replication
RAD30	YDR419W	Bypass synthesis DNA polymerase
NTG1	YAL015C	Base excision repair

Wild type: strain BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0).
RDKY3590 (MATa, ura3-52, leu2D1, trp1D63, hom3-10; lys210A).