Antigenicity and Immunogenicity of Rotavirus VP6 Protein Expressed on the Surface of Lactococcus lactis
Figure 1
Expression analysis of VP6-CWA. (a) Coomasie-blue-stained SDS-PAGE of L. lactis NZ9000/pCWA:VP6 total protein extracts at different times after induction. (b) Western blot analysis of induced L. lactis NZ9000/pCWA:VP6 total protein extracts at different times after induction to detect VP6 with a polyclonal serum against rotavirus. Rotavirus particles protein extract was included as a positive control (RV). The sizes of molecular weight standards (MWS) (in kilodaltons) are indicated between the gel and the membrane and the expected sizes of the VP6-CWA or VP6 band are denoted on the left or right of the gel or membrane, respectively. ON: overnight incubation.
