Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation

Table 1

Target regions, primer sequences, and size of amplified fragments.

Region	Target	Primer sequences	Product size (bp)
PIN3	Intron 3	5^’ GGGACTGACTTTCTGCTCTTGT 3^’	147 or 163
		5^’ GGGACTGTAGATGGGTGAAAAG 3^’
Region 1	Exon 4	5^’ TTGCCGTCCCAAGCAATGGATGAT 3^’	199
		5^’ TCTGGGAAGGGACAGAAGATGAC 3^’
Region 2	Exon 5 and 6	5^’ GCCGTCTTCCAGTTGCTTTA 3^’	488
		5^’ TAACCCCTCCTCCCAGAGAC 3^’
Region 3	Exon 7	5^’ TTGGGCCTGTGTTATCTCCT 3^’	253
		5^’ TGATGAGAGGTGGATGGGTAG 3^’
Region 4	Exon 8	5^’ CAAGGGTGGTTGGGAGTAGA 3^’	331
		5^’ TGCTAGGAAAGAGGCAAGGA 3^’