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BioMed Research International
Volume 2013 (2013), Article ID 310691, 8 pages
Research Article

Stat3 Upregulates Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 4 Expression in Osteosarcoma Cells

1Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai 200032, China
2Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Received 26 September 2013; Revised 15 November 2013; Accepted 17 November 2013

Academic Editor: Brian Oliver

Copyright © 2013 Jia Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

LGR4 expression was not changed by TNFa or IL-1a treatment (Fig.S1). Overexpression of CA-Stat3 promoted cell proliferation in MG63 and U2OS cells (Fig.S2). The mRNA and protein levels of LGR4 were increased in osteosarcoma tissues from patients, which is well-correlated with the activation of Stat3 signaling, as evidenced by its phosphorylation (Fig.S3). The mRNA levels of Cyclin D1 and Cyclin E, two downstream target genes ofb-catenin, were also reduced in cells with Lgr4 deficiency (Fig.S4).

  1. Supplementary Material