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BioMed Research International
Volume 2013 (2013), Article ID 316304, 8 pages
http://dx.doi.org/10.1155/2013/316304
Research Article

Oral Immunogenicity of Plant-Made Mycobacterium tuberculosis ESAT6 and CFP10

1Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia
2State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Oblast 630559, Russia
3National Center of Biotechnology, ul. Valikhanova 13/1, Astana 010000, Kazakhstan

Received 12 June 2013; Accepted 28 November 2013

Academic Editor: Armando Acosta

Copyright © 2013 Elena A. Uvarova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Two lines of transgenic carrot plants producing Mycobacterium tuberculosis proteins (ESAT6 and CFP10) have been constructed. The target proteins are present in carrot storage roots at a level not less than 0.056% of the total storage protein (TSP) for ESAT6 and 0.002% of TSP for CFP10. As has been shown, oral immunization of mice induces both the cell-mediated and humoral immunities. These data suggest that the proteins in question are appropriate as a candidate edible vaccine against tuberculosis.