Oral Immunogenicity of Plant-Made Mycobacterium tuberculosis ESAT6 and CFP10

Figure 6

LBTT for the immunized animals after stimulation with the mitogen PHA: (1) animals orally immunized with the recombinant carrot (5 g) producing the ESAT6 protein; (2) animals orally immunized with the recombinant carrot (5 g) accumulating producing the CFP10 protein; (3) animals immunized with the rESAT6 protein (subcutaneously 100 ng); (4) animals immunized with the rCFP10 protein (subcutaneously 100 ng), and (5) animals of the pooled control group (control 1—nontransgenic carrot, orally, and control 2—PBS solution, subcutaneously).