Figure 1: KJ1.26+ CD4+ T cell purification and Th1 polarization of DO11.10 cells. CD4+ T cells from spleens of DO11.10 BALB/c TCR Tg mice were purified: (a) 97.88% were CD4+ and (b) 91.88% were KJ1.26+ CD4+. Th1 polarization, then, was induced in purified cells, (c) IFN-γ levels were measured in the supernatant of cocultures of DO11.10 CD4+ T cells, and macrophages, IL-12, and OVA323-339 peptide were used. * , T-test.