Development of Pineapple Microsatellite Markers and Germplasm Genetic Diversity Analysis

Table 2

Sequences of the adapters and primers.

Name of primers	Sequences of primers (5′-3′)
PstI adapter	Sense strand: CTC GGA AGC CTC AGT CCC AGA CTG CGT ACA TGC A-OH
	Antisense strand: phos-TGT ACG CAG TCT GGG ACT GAG GCT TCC GAG A-OH
MseI adapter	Sense strand: GAG CAA GGC TCT CAC AAG GAC GAC CGA CGA G-OH
	Antisense strand: phos-TAC TCG TCG GTC GTC CTT GTG AGA GCC TTG CT-OH.
MseI suppressed amplification primer	GAG CAA GGC TCT CAC A
PstI suppression amplification primer	CTC GGA AGC CTC AGT C
MseI preamplification primer	GAC GAC CGA CGA GTA AC
PstI preamplification primer	AGA CTG CGT ACA TGC AGG A
PstI SAM primers	Adapter 1: AGA CTG CGT ACA TGC AGG ACC
	Adapter 2: AGA CTG CGT ACA TGC AGG ACG
	Adapter 3: AGA CTG CGT ACA TGC AGG AGC
	Adapter 4: AGA CTG CGT ACA TGC AGG A CT
	Adapter 5: AGA CTG CGT ACA TGC AGG A TC
	Adapter 6: AGA CTG CGT ACA TGC AGG A CA
	Adapter 7: AGA CTG CGT ACA TGC AGG A AC
	Adapter 8: AGA CTG CGT ACA TGC AGG A GT
	Adapter 9: AGA CTG CGT ACA TGC AGG A TG
	Adapter 10: AGA CTG CGT ACA TGC AGG A GA
	Adapter 11: AGA CTG CGT ACA TGC AGG A AG
	Adapter 12: AGA CTG CGT ACA TGC AGG A AT
5′-Anchored SSR primers	PAC: a: KKR YRY YAC ACA CAC ACA C
	b: KKY RYR YCA CAC ACA CAC A
	PCT: a: KKV RVR VCT CTC TCT CTC T
	b: KKR VRV RTC TCT CTC TCT C

K = G/T, R = G/A, Y = T/C, V = G/C/A, H = A/C/T for 5′-anchored SSR primer sequences.