Development of Pineapple Microsatellite Markers and Germplasm Genetic Diversity Analysis

Table 5

Details of the SSR primers for genetic diversity analysis.

Prime Pairs	Sequence ID	SSR motif	Forward primer (5′-3′)	Reverse primer (5′-3′)	Expected product size (bp)
Bp-01	AC 1.3	(CA)8	TCACACACACACACACAAAAAC	ATGGATTGCGCTGAGCTG	119
Bp-08	AC 6.3	(TG)8	ATGATGCCAGTGGAGTGTTC	ACACACACACACTTTTCTCATTG	152
EP-02	DT339694	(GAA)7	CGTGCCGCATAAATCAT	TATCTCCTCGCTCCTCTTG	116
EP-05	DT339172	(CCAT)8⋯ (AT)7	CAGCCAATAACAACCTCAAG	TCCATACACACAGTACGTCG	263
EP-06	DT339094	(CTTTTT)5	CGACTCGAGGATTACATTACG	GAGCACAAAGAACCACACAG	270
EP-09	DT338799	(TC)19	CCGAGGAAGAAGAAGAGGT	GGTCCACAGTTGTTTCAGTT	160
EP-10	DT338783	(GAT)7	GACCTTTATCCATCGCATC	CCATCAAACGTGAAATCTTG	266
EP-11	DT338752	(CAGGAG)5	AGCGAGATAGCAGAGATAGG	TAGAGCGATGTTCGGATG	180
EP-12	DT338506	(AG)6⋯ (AG)6	TTAACACATGCACGGAGTAC	CTAAGAGACAACCCAGGAAG	236
EP-13	DT338494	(CCAT)8⋯ (AT)8	GCCAATAACAACCTCAAGC	TCCATACACACAGTACGTCG	263
EP-15	DT338171	(GCAGTC)7	ACCTACAAGTGGTACGTCG	GGAGCAAGGAGTTATTCAG	242
EP-16	DT338171	(TC)6⋯ (AG)18	TAGTGAGTCAGGAGGAGAATG	CAAATAAACGGAGCGGAT	212
EP-20	DT337383	(TCT)8	TAATCGGGTGGAGTAAGG	GCTCACATAGGCCAATATG	155
EP-23	DT337096	(TC)20	ATGGTGGTTCACTTATCAGC	AGACATTCAAAGCGGAGAG	126
EP-24	DT337054	(CT)10	GCTGCTCTTGCTGCCAT	AAGCCATAGGACCACCAC	166
EP-26	DT336292	(AT)8	GAAGCGCAGGTTCGTAAT	ACAGAAGTAGAGGAAAGCAGC	227
EP-27	DT336032	(TCT)6	ATACTCTGCTGCTGTGAACG	TTGCACTCCTCTTTGCTAAC	155
EP-29	CO731867	(AGC)9	GCGAGCCTGTTAGACTTTGT	ACGATCTCAGCTGGACCTT	213