Association of Toll-Like Receptor 4 Polymorphisms with Diabetic Foot Ulcers and Application of Artificial Neural Network in DFU Risk Assessment in Type 2 Diabetes Patients

Table 2

Primers for PCR-RFLP of the TLR4, restriction enzymes used, and base pair products for genotypes.

SNP ID	Forward primer	Reverse primer	Restriction enzyme used	Product size and genotypes
rs4986790	CTGCTCTAGAGGGCCTGTG	TTCAATAGTCACACTCACCAG	BccI	140 = AA; 140, 77, 63 = AG; 77, 63 = GG
rs4986791	CTACCAAGCCTTGAGTTTCTG	AAGCTCAGATCTAAATACT	BslI	110 = TT; 110, 89, 22 = TC; 89, 22 = CC
rs11536858	ATAACCTCAGTGGGCTCTGG	ATGTTCTGGCATCTGGGAAG	KpnI	241 = AA; 241, 190, 51 = AG; 190, 51 = GG
rs1927911	TCACTTTGCTCAAGGGTCAA	AAACCTGCATGCTCTGCAC	StyI	203 = TT; 203, 178, 25 = TC; 178, 25 = CC
rs1927914	ACAAAATGGTCCCTCACAGC	TGGAAAGTAGCAAGTGCAATG	SphI	150 = TT; 157, 90, 67 = TC; 90, 67 = CC