[Retracted] Construction and Immunogenicity of DNA Vaccines Encoding Fusion Protein of Porcine IFN-𝝀1 and GP5 Gene of Porcine Reproductive and Respiratory Syndrome Virus

Figure 3

Expression of the fusion protein which encoded by the recombinant plasmid pcDNA3.1-PoIFN-λ1-SynORF5 in the transfected cells. Approximately 70–80% confluent Hela cells were tansfected with 2 μg of pcDNA3.1-PoIFN-λ1-SynORF5 (lane 3), pcDNA3.1-SynORF5 (lane 2) and control vector pcDNA3.1 (+) (lane 1), respectively. At 48 h after transfection, the cells were collected and subjected to Western blot as described in Section 2. Protein standards are indicated on left side of panel.