Regulatory Interactions between Androgens, Hoxb5, and TGFβ Signaling in Murine Lung Development

Figure 4

Effect of TGFβ inhibition on lung histology and Hoxb5, SMAD2P, and SMAD7 spatial and cellular expression. Cultured E11 fetal mouse lungs treated with control antibody had similar staining for SMAD2P (a), SMAD7 (b), and Hoxb5 (c) as untreated controls (Cf. Figure 2). Inhibitory TGFβ antibody (1d11) treatment led to formation of immature appearing airways that mostly had narrow lumens and decreased epithelial (↑) and mesenchymal (*) SMAD2P immunolocalization (d), whereas SMAD7 (e) was relatively unchanged. Hoxb5 (f) mesenchymal localization appeared more diffuse with TGFβ antibody treatment. Figures are representative tissue sections from at least 3 lungs per condition from 3 experiments; 100x Mag.