A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Sixteen Human Respiratory Virus Types/Subtypes
Table 2
Performance of the two-tube assay for individual target compared with the Luminex xTAG RVP Fast assay.
Viruses
No. of specimens: two-tube/Luminex xTAG RVP Fast assay
Performance of the two-tube assay compared with the Luminex xTAG RVP Fast assay
+/+
+/−
−/+
−/−
Sensitivity%
Specificity%
PPV%
NPV%
Accordance rate%
Kappa
FluA
20
0
0
227
100
100
100
100
100
1.000
sH1N1
14
0
2
231
87.50
100
100
99.14
99.19
0.929
FluB
1
0
0
246
100
100
100
100
100
1.000
PIV1
1
0
0
246
100
100
100
100
100
1.000
PIV2
2
0
0
245
100
100
100
100
100
1.000
PIV3
22
2
0
223
100
99.11
91.67
100
99.19
0.952
HRV
95
15
13
124
87.96
89.21
86.36
90.51
88.66
0.770
HMPV
27
1
0
219
100
99.55
96.43
100
99.60
0.980
Adv
24
11
1
211
96.00
95.05
68.57
99.53
95.14
0.773
CoV NL63
1
0
0
246
100
100
100
100
100
1.000
CoV OC43
11
0
0
236
100
100
100
100
100
1.000
CoV 229E
2
0
0
245
100
100
100
100
100
1.000
CoV HKU1
3
0
0
244
100
100
100
100
100
1.000
RSVA
7
1
0
239
100
99.58
87.50
100
99.60
0.931
RSVB
86
10
0
151
100
93.79
89.58
100
95.95
0.913
HBoV
28
0
5
214
84.85
100
100
97.72
97.98
0.907
The numbers of positive and negative specimens detected by both testing methods are shown. This table shows the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and the kappa values for each target using the Luminex xTAG RVP Fast assay as the reference for comparison. All the kappa values were above 0.75, indicating that the two assays were in agreement.
