A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Sixteen Human Respiratory Virus Types/Subtypes

Table 3

The confirmed results for specimens with discordant results between the two-tube assay and the Luminex xTAG RVP Fast assay.

Case no.	Two-tube	Luminex xTAG RVP Fast^a	Seeplex	Confirmed results
43	HRV	HEV/HRV, HBoV	HRV	HRV
349	HRV, RSVB	HEV/HRV, RSVB, and HBoV	HRV, RSVB	HRV, RSVB
352	HRV, RSVB	HEV/HRV, RSVB, and HBoV	HRV, RSVB	HRV, RSVB
146	HMPV	HMPV, HBoV	HMPV	HMPV
149	HMPV	HMPV, HEV/𝐇𝐑𝐕^b, and HBoV	HRV, HMPV	HRV, HMPV
16	None	HEV/HRV	HRV	HRV
125	RSVB	RSVB, HEV/HRV	HRV, RSVB	HRV, RSVB
262	RSVB, PIV3, and HBoV	RSVB, PIV3, HBoV, and HEV/HRV	PIV3, RSVB	RSVB, PIV3, and HBoV
270	PIV3, RSVB, and HMPV	PIV3, RSVB, HMPV, and HEV/HRV	PIV3, RSVB, and HMPV	PIV3, RSVB, and HMPV
142	RSVB	RSVB, HEV/HRV	RSVB	RSVB
21	PIV3	PIV3, HEV/HRV	PIV3	PIV3
66	None	HEV/HRV	None	None
85	FluA, sH1N1, and HBoV	FluA (H1), HBoV, and HEV/HRV	FluA	FluA, sH1N1, and HBoV
87	None	HEV/HRV	None	None
91	None	HEV/HRV	None	None
114	None	HEV/HRV	None	None
274	HRV, RSVB	HEV/HRV, RSVB, and Adv	HRV, Adv	HRV, Adv, and RSVB
28	FluA, HBoV, and 𝐀𝐝𝐯^c	FluA ( 𝐇𝟏^c ), HBoV	ND^d	FluA, sH1N1, HBoV, and Adv
118	FluA, Adv, 𝐑𝐒𝐕𝐀^c, 𝐑𝐒𝐕𝐁^c, and HBoV	FluA ( 𝐇𝟏^c ), HEV/HRV, Adv, and HBoV	FluA, RSVA, Adv, and HBOV	FluA, sH1N1, Adv, RSVA, RSVB, and HBoV

aViruses with discordant results are shown in boldface.
bThe Luminex xTAG RVP Fast assay was not able to distinguish rhinovirus from enterovirus, so HRV positives are described as HEV/HRV.
cThe Adv in no. 28 specimen, the RSVA, RSVB in no. 118 specimen, and the FluA subtype H1 were confirmed by sequencing.
dND stands for not detected.