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BioMed Research International
Volume 2013, Article ID 349408, 9 pages
http://dx.doi.org/10.1155/2013/349408
Research Article

Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen

1Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Hezar Jerib Street, Isfahan, Iran
2Department of Medical Physics, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
3Department of Immunology, Medical School, Isfahan University of Medical Sciences, Hezar Jerib Street, Isfahan, Iran
4Department of General, Organic, and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, 19 Avenue Maistriau, 7000 Mons, Belgium
5Micromod Partikletechnologie GmbH, Rostock, Germany

Received 14 September 2012; Accepted 5 December 2012

Academic Editor: Susana N. Diniz

Copyright © 2013 Daryoush Shahbazi-Gahrouei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis.