Asymmetry of the Active Site Loop Conformation between Subunits of Glutamate-1-semialdehyde Aminomutase in Solution
Figure 3
Fluorescence emission spectra of PLP-GSAM and PMP-GSAM. To allow direct comparison of the data, the reported spectra are normalized for protein concentration. Spectra were collected in 20 mM Tricine buffer, pH 7.9 solutions and were normalized to a protein concentration of 7 μM. (a) Emission spectra of PLP-GSAM (solid line) and PMP-GSAM (dashed line) upon excitation at 298 nm with 3 nm emission and excitation slits. (b) Emission spectra of PLP-GSAM (solid line) and PMP-GSAM (dashed line) upon excitation at 420 nm with 5 nm emission and excitation slits. (c) Emission spectra of PLP-GSAM upon excitation at 320 nm (solid line), 330 nm (dotted line), and 340 nm (dashed line) with 3 nm emission and excitation slits. (d) Emission spectra of PMP-GSAM upon excitation at 320 nm (solid line), 330 nm (dotted line), and 340 nm (dashed line) with 3 nm emission and excitation slits.
