Growth Arrest on Inhibition of Nonsense-Mediated Decay Is Mediated by Noncoding RNA GAS5
Figure 1
UPF1-specific siRNA increases cell death and inhibits cell proliferation in the human T-cell lines Jurkat and CEM-C7 and in the B-lymphoblastoid cell line BJAB. (a) Cells were transfected with 3 different specific UPF1 siRNAs or negative control siRNA ((−)siRNA) and cultured at 37°C. After 72 h the expression of endogenous UPF1 in the siRNA transfected cells was determined by real-time RT-PCR (mean s.e.m. from three separate experiments) and expressed relative to untransfected controls. ((b)–(d)) Cells were transfected with UPF1-siRNA1 or negative control siRNA ((−)siRNA) and cultured at 37°C. Viable cell numbers were determined after 48 h (b) and 72 h (c) by the LIVE/DEAD assay (Section 2.2). Results are represented as mean s.e.m. from three independent experiments. (d) Cell proliferation was measured after 48 h using the BrdU colorimetric ELISA assay. Results are represented as mean s.e.m. from three independent experiments. * compared with (−)siRNA.
