Activation of the ERK1/2 Signaling Pathway during the Osteogenic Differentiation of Mesenchymal Stem Cells Cultured on Substrates Modified with Various Chemical Groups

Figure 4

Real-time PCR analysis of the expression of osteogenesis-related genes in BMSCs cultured on surfaces modified with different chemical functional groups. (a) CBFA-1; (b) ALP. ^☆𝑃<0.05 versus the respective –CH₃-modified substrate at 7 d; ^◆𝑃<0.05 versus the respective –CH₃-modified substrate at 10 d; ^∗𝑃<0.05 versus the respective –CH3-modified substrate at 14 d.

(a)

(b)