Figure 8: The –NH2-modified substrate induces the activation of ERK1/2 in BMSCs. Cells were cultured on the –NH2-modified substrate (a) and the –CH3-modified substrate (b), and lysates were prepared at the indicated times. Lysates were subjected to immunoblot analysis using phosphospecific and nonactivated ERK1/2 kinase antibodies. An aliquot of each lysate was subjected to a kinase detection assay using SDS-PAGE. GAPDH was used as a control.