BioMed Research International / 2013 / Article / Fig 4

Research Article

Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances

Figure 4

The number (a, c, e, and f) and the size of the spreading area (b, d) of rat aortic smooth muscle cells in cultures on nonmodified LDPE (pristine), on LDPE irradiated with plasma (plasma), on LDPE irradiated with plasma and grafted with glycine (Gly), on polyethylene glycol (PEG), on bovine serum albumin (BSA), on colloidal carbon particles (C), or on bovine serum albumin and C (BSA+C). A tissue culture polystyrene dish (PS dish) was used as a reference material. Days 1, 2, 5, and 7 after seeding. Mean ± SEM from 3 samples, each measured 50 times (cell number, Vi-CELL Analyser) or from 119 to 229 cells for each experimental group (spreading area). ANOVA, Student-Newman-Keuls method. Statistical significance: *,# compared to the value on pristine PE and a polystyrene dish, respectively.
(a) Cell number, day 1
(b) Spreading area, day 1
(c) Cell number, day 2
(d) Spreading area, day 2
(e) Cell number, day 5
(f) Cell number, day 7