Figure 4: Effect of edaravone on oxidative stress following CCI. (a) Representative core- (upper) and peri-injury (lower) images of NT staining at 24 hours following CCI. Edaravone ( ) was injected at 0, 3, or 6 hours following CCI; vehicle ( ) treatment occurred at 0 hours. The inset shows a higher power view of the boxed region in the main image. The injury volume was calculated by integration of the TBI areas (based on seven 50 μm coronal sections at 500 μm intervals) and expressed as total (b) or core- and peri-injury (c) volumes. Data are expressed as mean ± SEM. Asterisk (*) indicates a significant difference between groups based on Tukey-Kramer tests ( values are described in the text).