Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology

Table 1

Sensitivity of methods for mutational analysis.

Methods	Limit of detection*
PCR/sequencing	10–25
Fragment analysis	5
Real-time PCR (allelic discrimination)	Up to 5
ARMS (Therascreen)	Up to 1
castPCR	Up to 0.1

∗Minimum percentage of mutant alleles in a wild type background required for reliable mutation detection.