BioMed Research International / 2013 / Article / Tab 1

Research Article

Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology

Table 1

Sensitivity of methods for mutational analysis.

MethodsLimit of detection*

PCR/sequencing10–25
Fragment analysis5
Real-time PCR (allelic discrimination)Up to 5
ARMS (Therascreen)Up to 1
castPCRUp to 0.1

Minimum percentage of mutant alleles in a wild type background required for reliable mutation detection.

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