Lignocellulosic Fermentation of Wild Grass Employing Recombinant Hydrolytic Enzymes and Fermentative Microbes with Effective Bioethanol Recovery
Figure 4
SSF profile of 1% (w/v) wild grass using GH5 cellulase, S. cerevisiae in shake flask. (●) cell OD measured at 600 nm, (▲) ethanol concentration (g·L−1), (▼) reducing sugar (g·L−1), and (○) specific activity (U·mg−1) with time (h). The cultivation conditions were 100 mL working volume in 250 mL Erlenmeyer flask, initial pH 4.3, temperature 30°C, and shaking 120 rpm.
