Natural Oncolytic Activity of Live-Attenuated Measles Virus against Human Lung and Colorectal Adenocarcinomas
Figure 1
In vitro oncolytic properties of measles virus against human adenocarcinomas. (a) One million human lung or colorectal adenocarcinoma cells were infected in vitro with a live-attenuated strain of MV recombinant for EGFP (MV-eGFP; MOI = 1). Percentages of infected cells (EGFP+) were determined by flow cytometry at 24, 48, and 72 h after infection. Data are presented as mean ± SEM, (). (b) Infected cells were observed for 72 h by time-lapse microscopy to study cytopathic effects of MV infection. Complete experiments are presented in Figures (Caco-2) and (A549) in Supplementary Material available online at http://dx.doi.org/10.1155/2013/387362. (c) Human lung and colorectal adenocarcinoma cells were either infected with live-attenuated MV (MOI = 1, black bars) as described above or left uninfected (white bars). Cells were cultured for 72 h without medium renewal. Cells were then double stained with annexin V/propidium Iodide and analyzed by flow cytometry.
