Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream

Table 5

Antioxidant activity of SFE extracts determined by DPPH. Results are expressed as EC₅₀ (g L⁻¹).

	Fraction
𝑃 (bar)	Pressure mode			𝑃 (bar)	Flow mode		Flow mode
	A	B	C		D	E	% modification	F	G
Cell	—	—	0.23	Cell	0.20	0.22	Cell	0.26	0.20
100	n.d.	2.65	37.73	100	0.33	0.74	0	21.99	0.64
120	n.d.	0.36	0.41	120	0.51	0.36	0.5	1.57	0.68
140	n.d.	0.27	0.25	140	0.62	0.23	1.0	0.59	3.52
160	n.d.	0.29	0.28	160	0.46	0.21	1.5	0.97	1.42
200	n.d.	0.34	1.00	200	0.74	0.22	2	0.88	0.80
250	n.d.	0.21	0.20	250	0.34	0.23	2.5	0.64	0.67
300	n.d.	0.10	3.12	300	0.42	0.34	3	0.54	0.42